Background: Modern healthcare systems increasingly depend on interdisciplinary collaboration to address complex medical needs, enhance patient outcomes, and improve service quality. Effective integration among clinical pharmacy, nursing, and medical laboratories is vital in optimizing treatment accuracy, medication safety, and patient-centered care. However, challenges such as role ambiguity, communication gaps, and resource constraints often limit the success of these collaborations. Aim: This study investigates the role of interdisciplinary integration between clinical pharmacy, nursing, and medical laboratories in enhancing healthcare quality. It also identifies barriers and facilitators that influence the effectiveness of such collaboration. Methods: A systematic review was conducted on peer-reviewed articles published between 2000 and 2016. The search included databases such as PubMed, Scopus, and ScienceDirect, utilizing keywords like “interdisciplinary collaboration,” “clinical pharmacy integration,” and “nursing and laboratory coordination.” Studies were selected based on their focus on collaborative practices in healthcare and their measurable impact on outcomes. Results: The analysis revealed that interdisciplinary integration significantly improves patient safety, reduces medication errors, and enhances diagnostic accuracy. Effective collaboration is fostered by clear communication protocols, mutual respect among professionals, and the use of advanced digital systems for data sharing. Conversely, barriers such as insufficient training, hierarchical dynamics, and resource limitations were noted.