This study aims to develop android Augmented Reality application as a learning media of human excretory system concept and to examine its practicality for biology students and teachers. This development research includes 3 stages, namely preliminary research, prototyping stage, and systematic reflection and documentation. The data were collected through interview to 100 students and 3 teachers, and questionnaires to 100 students and 3 teachers from three schools. This study has resulted in Android AR application for human excretory system concept which is rated “good” by expert review, small group evaluation and field study, and “very good” through one-to-one evaluation. This application is interesting for and is accepted by students and teachers as an alternative learning media for human excretory system concept, and is effective to help them in biology teaching-learning process.