Tuberculosis (TB) remains a major global health problem. T-cells mediated immunity contribute to protection from Mycobacterium tuberculosis infection. CD4+ and CD8+ T-cells secreted interferon gamma (IFN-γ) to stimulate fusion of phagolyzosome and produce free radicals for killing mycobacteria. Early secretory antigenic target 6 kD(ESAT-6) and culture filtrate protein 10(CFP-10) are dominant M. tuberculosis antigens in inducing IFN-γ. This study compared CD4+ and CD8+T-cells expressing IFN-γ with and without ESAT-6- CFP-10 fusion antigen stimulation in active tuberculosis patients. Peripheral blood mononuclear cellsfrom active TB patients were cultured with and without ESAT-6-CFP-10 fusion antigen. CD4+ and CD8+ T-cells expressing IFN-γ were examined using flow cytometry.CD4+ T-cells expressing IFN-γ after antigen stimulation compare to controls(1.51%±1.7 vs 1.23%±0.86; p=0.08),and CD8+ T-cells (1.58%±0,99 vs 1.92%±1.13; p=0.86).CD4+ and CD8+T-cells expressing IFN-γ after antigen stimulation (1.51%±1.7 vs 1.58%±0,99; p=0.94).There were no significant differences of CD4+ and CD8+T-cells expressing IFN-γ after ESAT-6-CFP-10 fusion antigen stimulation in active pulmonary tuberculosis patients.